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From: Rothschild, Max F. Date: Fri, 02 Sep 94 09:14:00 CDTOur apologies. The editor made this tough to read. Hope it can be read now.PIG GENOME NEWS No. 8 September 1The ISAG meetings in Prague were excellent forums topresent a great deal of new results from pig genemappers.
Attendees at the conference included C. Louis(MN), J. Lunney (BARC), C. Rohrer, L.Alexander (MARC), B. Kirkpatrick (WI), M.
Tuggle (IA) and D. Frahm (CSRS). Most of the majorEuropean pig gene mappers were also there. Severalabstracts on pig gene mapping were presented includingthe PiGMaP linkage map, identification of newmicrosatellite markers, demonstrations of new physicalmapping techniques and results, QTL results andcomparative gene mapping in the pig. If you areinterested in a particular abstract please feel free tocontact me. The 2nd Pig Gene Mapping Workshop (PGM 2)was held also and attended by 79 participants.
Resultsfrom several groups were presented. The PiGMaP linkagemap contains 237 genes. Clay Center presented resultsthat their map now contains 667 markers. This makesthe total near 900 markers and genes. Most of the USDAmarkers (71%) are less than 5 cM from the next markerand the USDA estimate of the total size of the porcinegenome was approximately 2600 cM. The MARC group hopesto map an additional 300+ markers within the next 6months. The Nordic group also presented their map whichwill appear in Genetics in August.
As part of theworkshop I discussed the coordination efforts in theU.S. And also presented a review of the gene mappingresearch being done at the 11 U.S. Labs.Several decisions were made at the workshop followingdiscussions. Nomenclature will follow the humannomenclature.
Locus symbols for anonymous DNA markerswill not be assigned until they have been mapped.Confirmed status of markers and genes will follow rulesused in human mapping also. The workshop committee forthe next meeting will be Alan Archibald, MerteFredholm, Gary Rohrer, Joel Gellin and Max Rothschild.The 5th World Congress on Genetics Applied to LivestockProduction was held in Guelph and was extremely wellattended. Over 1200 delegates were there, including B.Kirkpatrick, J. Rothschild, A. Clutter, D.Pomp, J. Frahm from the U.S.
Swine genemapping group and several international pig genemappers. This meeting happens every 4 years and thisyear there was over a 50% increase in attendees. Ofspecial note was the increase in papers and attendancefor the gene mapping, genetic marker and polymorphismpapers. An overview of pig gene mapping was providedby Alan Archibald. We had a very good workshop on QTLsin which Chris Haley presented some of his thoughts.Of particular interest and excitement were the papersthat presented major genes and markers for traits.
Inpigs, there were two statistical papers presentingevidence for a major gene in Meishan pigs thatcontrolled intramuscular fat (marbling) and a gene thataffects production of Paris Hams. In terms ofmolecular work, two papers were presented. Evidencefor a major gene for litter size in pigs was presentedby M. Rothschild et al. This gene, the estrogenreceptor gene has been mapped to chromosome 1. Resultsdemonstrated a 1.5 to 2.1 pig advantage for animalsheterozygous or homozygous for the favorable allele.In another paper, Edfors-Lilja et al. (Sweden)presented evidence for a gene on the other end ofchromosome 1 which controls white blood cell count.Additional markers were found on chromosome 8 whichwere associated with other measures related to naturalimmunity.
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These results demonstrated that QTLdetection has come a long way since the last Congressfour years ago. The next Congress will be in Australiain 1998 or 1999.Database developments continue to move along nicely.The co-editorship between the PiGMaP (Archibald) andU.S. (Rothschild) coordinators of the pig database isworking well and over 200 publication entries will bein the database soon. Alan Archibald and myself mettwice, once with Alan Hillyard (at Prague) and oncewith Lizhen Wang (at Guelph). Some of the new changesin the database will be discussed at our Septembermeeting.
A large number of new users have signed upfor the database. At present, nodes for the databaseexist in the U.S., Scotland and New Zealand. Somediscussion was made to standardize the name of all thedatabase nodes to PiGBASE. This is a topic for our NC-210 meeting in September. In addition to the U.S.
Piggene mapping WWW Home page, the PIGBASE WWW Home pagehas been developed. Access to it is possible throughthe U.S. Pig gene mapping WWW.Some very good recent publications on databasedevelopment by the USDA Clay Center group (John Keele,Gary Rohrer, Craig Beattie et al.) have been publishedin a new journal, Journal of Computational Biology. Inaddition, the recent issue of Science (vol 265, August12, 1994) has some interesting papers on WWW andMosaic.The NAGRP meeting and the NC-210 meeting will be heldon September 22-23 in Minneapolis at the Radisson Hotel(phone: 612-379-8888, mention Animal Genome Project).Please plan to attend if you are a NC-210 member or aninterested industry person. NC-210 members are toprepare written reports as outlined in the materialJoan Lunney recently sent out. The NAGRP meeting looksquite good and there will be one speaker on the humangenome project and another from Applied Biosystems todiscuss new developments in technology. We owe a bigthanks to Dr.
Jerry Dodgson for arranging and planningthe meetings in Minneapolis. After this meeting willbe the Chromosome 6 Workshop. On October 1-3 will be ahuman genome meeting in Washington D.C. Other meetingdates and details will be presented in the newsletterin the future.
If you have a meeting to advertise orinterest in knowing about particular meetings, pleasecontact me as I will be attending several of them.Please note that I will no longer be using my 294-3629phone number. I can be reached at 515-294-6202. Ihave now left my half time job as Assistant Director inthe Iowa Agriculture Experiment Station and returned tothe Animal Science faculty full time. While the last 4years have been fun I have planned this move for sometime and it should give me more time for thecoordination efforts and my research.Contributions to Pig Genome Update 9 are alwayswelcome. Please send by the 20th of October.Hope to see many of you at the meetings in Minneapolis.Max F.
RothschildNational Pig Genome Coordinator225 Kildee HallDepartment of Animal ScienceIowa State UniversityAmes, Iowa 50011Phone: 515-294-6202 Fax: 515-294-2401cc: Dick Frahm, CSRS and Roger Gerrits, ARSFrom: 'Max Rothschild' To: angenmapatiastate.eduOrganization: College of Agriculture, Iowa StateDate: Wed, 13 Oct 1993 07:15:32 CSTSubject: thought to discussAngenmap discussants:We continue to add lots of quiet people to the discussion group. Howabout some thought to discuss?From: Ian.Hermansatvuw.ac.nzDate: Wed, 08 Dec 1993 17:10:00 +1300To: angenmapatiastate.eduMessage-Id: Subject: selective genotypingTo add more confusion to the discussion on selective genotyping.What statistical analysis should be applied to a DNA fingerprintband which appe ars to segregate with a multi-allelic trait on the basisof selective genotyping of extremes? Analyses have been within a single,large half-sib family (170 off spring). Any advice?Ian HermansSchool of Biological SciencesVictoria University of WellingtonWellingtonNew ZealandFrom: Ian.Hermansatvuw.ac.nzDate: Wed, 08 Dec 1993 17:10:00 +1300To: angenmapatiastate.eduMessage-Id: Subject: selective genotypingTo add more confusion to the discussion on selective genotyping.What statistical analysis should be applied to a DNA fingerprint band whichappe ars to segregate with a multi-allelic trait on the basis of selectivegenotyping of extremes? Analyses have been within a single, large half-sibfamily (170 offspring).